BBa_K1072007

BBa_K1072007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1072007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Junjie zhang, Yichong wang, Zewei yan, Rui chen and Xuenian chen

Date created: 2013-09-06 11:00:00

Date modified: 2015-05-08 01:09:01

Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+α
-ALS+2TM

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1072007_sequence (Version 1)

Description

BBa_K1072007 is a composite divece, including LuxR, LuxI, GFP and α-ALS genes.

LuxR is under control of a constitutive promotor, Lux pI. Produce, LuxR, forms a complex with the quorum sensing molecule AHL and this complex subsequently increases the transcriptional rate of the lux pR promoter while also decreas the transcriptional rate of the lux pL promoter. The other genes are under control of this lux pR promoter.

The generator, Lux pR+RBS+LuxI+B0015, express AHL which will combine with LuxR.

GFP also is under control of Lux pR, presenting as reporter.

α-ALS，as our desire produce, will transcrip activated by LuxR and AHL complex. If co-transforming with B0015 , this system will transcript α-ALS periodically.

Finally, this part just is the combination of BBa_K1072000 and BBa_K1072006 .

Notes

-

Source

Some part get from registry and α-ALS gene originate from Lactococcus lactis, respectively.

Components

Access	Instance	Definition
public	BBa_K1072000	BBa_K1072000
public	BBa_K1072006	BBa_K1072006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1072000	1,2910	BBa_K1072000
BBa_K1072006	2919,4801	BBa_K1072006

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1072007/1

Member of these Collections

• //cds/enzyme
• //chassis/prokaryote/ecoli
• //function/reporter/fluorescence
• iGEM Parts Registry