Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yichong wang, Rui chen, Xihao liao and Junjie zhang
Date created: 2013-08-30 11:00:00
Date modified: 2015-05-08 01:09:01
Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP.
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1072000_sequence (Version 1) |
Description
BBa_K1072000 three protein generators: lux pl-RBS-C0062-B0015 produces LuxR. LuxR forms a complex with the quorum sensing molecule AHL and this complex subsequently increases the transcriptional rate of the lux pR promoter while also decreasing the transcriptional rate of the lux pL promoter. Both of the other generators are under control of this lux pR promoter.The second generator ???luxpR-RBS-J04031-B0015???has a low basal expression of the GFP, which can - of course - be monitored.
The third generator produces luxI, which produces the quorum sensing molecule AHL. In combination with LuxR, AHL thus increases the production of AHL and GFP.
This part is an improvement of BBa_K546000 and K546005 made by 2011 iGEM11_Wageningen_UR team. A LuxpR promoter, RBS, GFP and double terminator has been added.