Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: M. Hesselman
Date created: 2011-09-13 11:00:00
Date modified: 2015-05-08 01:12:39
Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.
| Types | DnaRegion |
| Roles | Signalling engineered_region |
| Sequences | BBa_K546000_sequence (Version 1) |
Description
The F2621 BioBrick device contains a lux pL regulated luxR repressor/activator and a lux pR promoter that is linked to an 3OC6HSL, an AHL autoinducer molecule, synthetase. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. Complementary to the lux pL promoter, the lux pR promoter with a complex of luxR and 3OC6HSL will enable enlarged activation of the downstream synthetase gene, thus increase the concentration 3OC6HSL.Notes
The original R0063 BioBrick part (promoter) of the F2621 BioBrick device turned out to be absent in our construct, as was shown by a sequence analysis. To increase the transcription activation there has been put an extra R0063 BioBrick part promoter in front of the F2621 BioBrick device. The resulting construct worked a lot better than the original F2621 - K082014 BioBrick system.Source
The lux p promoters and the autoinducer producing enzyme originate from the quorum sensing ability of Vibrio fischeri.| Access | Instance | Definition |
| public public | BBa_F2621 BBa_K082014 | BBa_F2621 BBa_K082014 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_F2621 BBa_K082014 | 1,1158 1167,1964 | BBa_F2621 BBa_K082014 |