Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Junqi kuang, Yaqing du and Xihao liao
Date created: 2013-09-09 11:00:00
Date modified: 2015-05-08 01:09:01
GAL1 + Brho +Flag + odr-10 +GFP +ADH1
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1072015_sequence (Version 1) |
Description
This is a GPCR generator of Odr-10 linking Brho signal peptide and reporter, flag tag and GFP.BBa_K1072015 encodes a diacetyl-sensing G-protein coupled receptor, 7-transmembrane receptors, which can be functionally expressed in S. cerevisiae.
This part is an improvement of BBa_K211002 and BBa_K775003 made by 2009 Hongkong university and 2012 TU delft team, respectively. A Brho signal peptide， flag tag and GFP have been added.
Notes
-Source
Caenorhabditis elegans olfactory receptor Odr-10 and kit plate| Access | Instance | Definition |
| public public public public public public | BBa_J63006 BBa_K1072009 BBa_K1072008 BBa_K1072010 BBa_E0040 BBa_K801012 | GAL1 BBa_K1072009 BBa_K1072008 BBa_K1072010 GFP BBa_K801012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J63006 BBa_K1072009 BBa_K1072008 BBa_K1072010 BBa_E0040 BBa_K801012 | 1,549 556,615 622,648 655,1674 1681,2400 2409,2757 | GAL1 BBa_K1072009 BBa_K1072008 BBa_K1072010 GFP BBa_K801012 |