Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Braunschweig 2013
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:01
Inducible ampicillin resistance cassette + RhlR expression cassette
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1073028_sequence (Version 1) |
Description
In complex with the inducer N-butyryl-HSL, the activator RhlR binds to the Rhl promoter and enables expression. This construct provides all the parts required to express the gene behind the rhl promoter. Only the inducer N-butyryl-HSL is missing, which usually is taken up from the media. By the regulation of the inducer's concentration in the media, the expression rate of the gene of interest (in this case ampicillin beta-lactamase) can be controlled.Notes
no considerations.Source
The biobricks for this part were taken from the 2013 distribution kit.| Access | Instance | Definition |
| public public public | BBa_K1073011 BBa_B0015 BBa_K1073014 | BBa_K1073011 BBa_B0015 BBa_K1073014 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1073011 BBa_B0015 BBa_K1073014 | 1,944 953,1081 1090,1938 | BBa_K1073011 BBa_B0015 BBa_K1073014 |