Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Braunschweig 2013
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:02
Autoinducer synthase LasI + LVA with combined eforRed chromoprotein expression cassette
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1073032_sequence (Version 1) |
Description
This part is the basis for the expression of the Las auto inducer synthetase, which synthesizes LasI [N-(3-oxododecanoyl)-homoserine lactone]. LasI binds to the activator LasR. Together they activate the Las promoter. A constitutive expression of the chromoprotein eforRed is added to the construct in order to varify the expression of LasI synthetase. The construct has no promoter in front of the LasI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)Notes
no considerations.Source
The biobricks for this part were derived from the 2013 distribution kit and the iGEM Team Uppsala.| Access | Instance | Definition |
| public public public | BBa_K1073018 BBa_K1073022 BBa_B0015 | BBa_K1073018 BBa_K1073022 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1073018 BBa_K1073022 BBa_B0015 | 1,823 832,1574 1583,1711 | BBa_K1073018 BBa_K1073022 BBa_B0015 |