BBa_K1073032

BBa_K1073032 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1073032

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM Team Braunschweig 2013

Date created: 2013-09-16 11:00:00

Date modified: 2015-05-08 01:09:02

Autoinducer synthase LasI + LVA with combined eforRed chromoprotein expression cassette

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1073032_sequence (Version 1)

Description

This part is the basis for the expression of the Las auto inducer synthetase, which synthesizes LasI [N-(3-oxododecanoyl)-homoserine lactone]. LasI binds to the activator LasR. Together they activate the Las promoter. A constitutive expression of the chromoprotein eforRed is added to the construct in order to varify the expression of LasI synthetase. The construct has no promoter in front of the LasI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)

Notes

no considerations.

Source

The biobricks for this part were derived from the 2013 distribution kit and the iGEM Team Uppsala.

Components

Access	Instance	Definition
public	BBa_K1073018	BBa_K1073018
public	BBa_K1073022	BBa_K1073022
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1073018	1,823	BBa_K1073018
BBa_K1073022	832,1574	BBa_K1073022
BBa_B0015	1583,1711	BBa_B0015

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1073032/1

Member of these Collections

• iGEM Parts Registry