BBa_K1076001

BBa_K1076001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1076001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luna Lacerda, Adolfo Mota, Carlos Gustavo Nunes
Date created: 2013-09-12 11:00:00
Date modified: 2015-05-08 01:09:03

Flanking genes for FadR deletion



Types
DnaRegion

Roles
sequence_feature

Other

Sequences BBa_K1076001_sequence (Version 1)

Description

Part of the upstream and dowstream regions of the gene FadR, containing part the genes SpoVR and NhaB respectively.This parts have Nde1 complementary site and they were ligate together. This method garantee generation of a deleted copy of the target gene using a two step assymmetric/crossover PCR amplification. The target in this case is the FadR. In other words its a system to knock down the FadR gene which is transcribed into a regulatory intake fatty-acid protein.

Notes

Design PCR primers to amplify the regions flanking the gene FadR. Subsequent to amplification, these flanking regions are fused together via a complementary ???tag??? region. This fusion product will then be inserted into the suicide vector pNPTS138.

Source

From genomic sequence

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BBa_K1076001/1