BBa_K1083001

BBa_K1083001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1083001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: David Jullesson

Date created: 2013-09-26 11:00:00

Date modified: 2015-05-08 01:09:04

HEWL-Antigen-Luciferase

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K1083001_sequence (Version 1)

Description

This generates a protein construct containing a epitope for HEWL (see biobrick BBa_K1083000). The HEWL epitope (V) is exchangebla due to a built in restriction site for XhoI. H and CH2 are optimezed for E.coli. The VHCH2 generates an antibody from cameloids. This part is connected to Lucifarese (biobrick BBa_K3255209).

Notes

This part was very tricky to design due to allot of restriction sites high gc content and not optimized for e. coli.

Source

This sequence comes from cameloids.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
HEWL-Antibody	1,1075	feature/protein, CDS
Luciferase	1076,2693	feature/protein, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1083001/1

Member of these Collections

• iGEM Parts Registry