BBa_K1085000

BBa_K1085000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1085000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Claudio Tiecher

Date created: 2013-08-29 11:00:00

Date modified: 2016-02-10 01:07:41

RBS Start-codon Strep-tag SpiderSilkSubunitE1

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1085000_sequence (Version 1)

Description

The part contains the coding sequence for part of the spider silk protein together with Bacillus Subtilis ribosome binding site (RBS) and the Strep-tag.

Notes

The DNA sequence was codon-optimized for Bacillus Subtilis.
Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.

Source

The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008).
The DNA sequence was synthesized by Integrated DNA Technologies (IDT).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RBS	1,6	ribosome_entry_site, feature/rbs
Strep	17,40	feature/tag, tag
spidersilk subunit E1	59,268	feature/cds, CDS
starting codon (ATG)	14,16	start_codon, feature/start

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1085000/1

Member of these Collections

• //cds
• //chassis/prokaryote/bsubtilis
• //collections/silk
• iGEM Parts Registry