Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Claudio Tiecher
Date created: 2013-09-09 11:00:00
Date modified: 2016-02-10 01:07:59
RBS Start-codon SpiderSilkSubunitE1
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1085001_sequence (Version 1) |
Description
This BioBrick contains the coding sequence for part of the spider silk protein (SubunitE1) with Bacillus Subtilis ribosome binding site (RBS) infront of it.The RBS is there to allow the ribosomes to bind and start to translate the DNA.
SubunitE1 codes for a spider silk protein that is optimized to prevent the forming of secundary RNA structures in close proximity to the RBS. This is given priority over reducing the number of restriction sites. However, the primary goal of the algorithm was still to optimize the codon selection based on their availbility scores.
Notes
The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.Source
The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008). The DNA sequence was synthesized by Integrated DNA Technologies (IDT).| Sequence Annotation | Location | Component / Role(s) |
| RBS spidersilk subunit E1 starting codon (ATG) | 1,6 17,226 14,16 | feature/rbs ribosome_entry_site feature/cds CDS feature/start start_codon |