Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Claudio Tiecher
Date created: 2013-09-09 11:00:00
Date modified: 2016-02-10 01:09:12
SpiderSilkSubunitE2 Strep-tag
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1085003_sequence (Version 1) |
Description
This BioBrick contains the coding sequence for part of the spider silk protein (SubunitE2) and a Strep-tag behind it.SubunitE2 codes for a spider silk protein that is optimized for maximal expression in Bacillus subtillus 168. The primary goal of the algorithm was to optimize the codon selection based on their availbility scores. The secondary goal was to minimize the number of restriction sites, and the tetrary goal was to prevent the formation of secondary RNA structures in close proximity to the RBS.
The amino acid sequence is based on the adapted MaSp2 sequences discribed in the paper of Brooks et. al. 2008.
The Strep-tag, fused at the C terminal, is used both as a binding tag to use to inidicate its production and as a binding component to biotin.
Notes
The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.Source
The amino acids sequence of the silk protein was obtained from the paper by Brooks et al. (2008). The DNA sequence was synthesized by Integrated DNA Technologies (IDT).| Sequence Annotation | Location | Component / Role(s) |
| strep spidersilk subunit E2 | 229,252 1,210 | tag feature/tag feature/cds CDS |