Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Claudio Tiecher
Date created: 2013-09-09 11:00:00
Date modified: 2015-06-02 12:11:00
SilkTail Strep-tag Stop-codon
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_K1085009_sequence (Version 1) |
Description
This BioBrick contains the coding sequence for silk tail with a Strep-tag behind it and ends with a stop codon.The Tail ??????.
The Strep-tag, fused at the C terminal, is used both as a binding tag to use to inidicate its production and as a binding component to biotin.
Notes
The DNA sequence was codon-optimized for Bacillus Subtilis. Moreover the nucleotide sequence was optimized in order to accomplish the production standard of IDT. A couple of optimized codons were affected by this.Source
The amino acid sequence of the tail was obtained from MaSp2. The DNA sequence was synthesized by Integrated DNA Technologies (IDT).| Sequence Annotation | Location | Component / Role(s) |
| strep spidersilk C-terminal domain Stop codon (TGA) | 289,312 1,270 313,315 | tag feature/tag feature/cds CDS stop_codon feature/stop |