BBa_K1086003

BBa_K1086003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1086003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: ??talo Faria do Valle

Date created: 2013-09-12 11:00:00

Date modified: 2015-05-08 01:09:06

TorCad promoter + RFP

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1086003_sequence (Version 1)

Description

This part is promoter of the TorCAD operon from Escherichia coli, and its expression only happens in response to Trimethylamine N-oxide (TMAO). The TMAO is a organic compound with the formula (CH3)3NO, and it is a metabolite produced by the liver in humans, but in bacteria TMAO is used to produce energy in the absence of oxygen. The TMAO binds to TorT protein, which activates the TorS protein. The TorS protein phosphorilates the TorR transcriptional factor, which binds to TorCAD promoter, activating the transcription.

Notes

This composite was made by the 3A protocol assembly

Source

One part of the composite comes from the genome sequence of Escherichia coli

Components

Access	Instance	Definition
public	BBa_K1086000	BBa_K1086000
public	BBa_J06702	mCherry

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1086000	1,77	BBa_K1086000
BBa_J06702	86,954	mCherry

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1086003/1

Member of these Collections

• iGEM Parts Registry