Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andreas Kj??r
Date created: 2013-08-19 11:00:00
Date modified: 2015-05-08 01:09:06
B. subtilis dxs-AmilCP fusion (constitutive promoter)
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1088006_sequence (Version 1) |
Description
This composite part consists of 5 sub partsA strong constitutive promoter: BBa_J23100
A strong RBS: BBa_J15001
The coding region of Dxs from B. sub: BBa_K1088000
A 10 aa Protein Linker: BBa_K105012
And the AmilCP chromoprotein: BBa_K592009
The idea was to detect expression of dxs protein by the reporter amilCP fused via a linker to the Dxs protein. Unfortunately it seems as though the amilCP protein does not work well as a protein-fusion reporter, and the color change was vague.
Notes
We added the linker, because we hoped it would improve the function of the chromoprotein amilCP. However it seems as though this did not work as intended. The promoter and RBS are as strong as we could find them, since we only where interested in knowing if the Dxs where expressed at all.Source
The parts used in this composite parts derives mainly from E. coli, however the amilCP derives from Acropora millepora.| Access | Instance | Definition |
| public public public public public | BBa_J23100 BBa_J15001 BBa_K1088000 BBa_K105012 BBa_K592009 | BBa_J23100 BBa_J15001 dxs (B. su BBa_K105012 amilCP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_J15001 BBa_K1088000 BBa_K105012 BBa_K592009 | 1,35 36,45 46,1947 1948,1977 1978,2646 | BBa_J23100 BBa_J15001 dxs (B. su BBa_K105012 amilCP |