Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andreas Kj??r
Date created: 2013-09-17 11:00:00
Date modified: 2015-05-08 01:09:06
HRT2 prenyltransferase from Hevea Brasilianis (ara promoter without araC: arabinose inducible)
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1088024_sequence (Version 1) |
Description
This part encodes the rubberproducing prenyltransferase from the rubber tree Hevea brasiliensis. It uses isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) as substrates to produce cis-1,4 polyisoprene natural rubber.The gene is expressed from the arabinose promoter (BBa_I13453), which is induceable with arabinose under glucose scarce conditions. Note that the regulater insn't a part of this part and thus no/very little transcription is expected.
Notes
There is a 3xFLAG-tag after the HRT2 stop codon, and thus the 3xFLAG-tag isn't translated.Source
Source is specified in components| Access | Instance | Definition |
| public public public public public | BBa_I13453 BBa_K1088021 BBa_J15001 BBa_K1088003 BBa_K1088025 | BBa_I13453 BBa_K1088021 BBa_J15001 HRT2 3xFLAG |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13453 BBa_K1088021 BBa_J15001 BBa_K1088003 BBa_K1088025 | 1,130 131,136 137,146 147,1001 1002,1067 | BBa_I13453 BBa_K1088021 BBa_J15001 HRT2 3xFLAG |