BBa_K1091005

BBa_K1091005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1091005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jian Huang
Date created: 2013-09-17 11:00:00
Date modified: 2015-05-08 01:09:07

pBAD-green fluorescent protein-SLOW



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1091005_sequence (Version 1)

Description

Part K1091005 is one of the CDS parts designed with the Transpeeder software version 1.0. Though it has an identical amino acid sequence with the wild type green fluorescent protein of Aequorea victoria, its nucleic sequence is designed to show more similarities with the Shine-Dalgarno sequence of E.coli via synonymous mutation using Transpeeder. The preliminary experimental results showed it had a slower translation speed than those of the wild type (K1091006) and fast parts (K1091004). Thus, the part is also called GFP-slow. Pay attention! This part is cloned in pBAD plasmid.

Notes

Its nucleic sequence is designed to show more similarities with the Shine-Dalgarno sequence of E.coli via synonymous mutation using the Transpeeder software version 1.0.

Source

It has an identical amino acid sequence with the wild type green fluorescent protein of Aequorea victoria.

igem#sampleStatus
It's complicated
igem#status
Planning
 
rdf-schema#seeAlso
P42212
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1091005/1