sfBFP

BBa_K1093000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1093000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jakub Piątkowski

Date created: 2013-07-21 11:00:00

Date modified: 2015-05-08 01:09:07

sfBFP

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1093000_sequence (Version 1)

Description

Superfolder Blue Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eBFP it displays improved stability and strong blue fluorescence.
Excitation peak: 380 nm
Emission peak: 440 nm

Notes

Y66H mutation was introduced into BBa_I746919 via site-directed mutagenesis

Source

sfBFP is based on superfolder Green Fluorescent Protein (BBa_I746919), with Y66H mutation

Reference
[1]P??delacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology, 24(1), 79???88. doi:10.1038/nbt1172

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start codon	1,3	start_codon, feature/start
stop codon	715,717	stop_codon, feature/stop
sfBFP	1,715	feature/protein, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1093000/1

Member of these Collections

• iGEM Parts Registry