Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakub Piątkowski
Date created: 2013-09-20 11:00:00
Date modified: 2015-05-08 01:09:08
HBA+NsfGFP (+B0034)
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1093020_sequence (Version 1) |
Description
Human hemoglobin alpha chain fused at C-terminus with the N-terminal fragment of superfolder green fluorescent protein. Along with BBa_K1093021 and BBa_K1093031 (hemoglobin beta chain fused with two variants of C-terminal of sfGFP) it is intended as a part of a reporter system for acrylamide detection. Formation of adducts on the N-terminal valine of both hemoglobin chains is known to ihibit proper assembly of hemoglobin tetramer.Part already contains a strong RBS (BBa_B0034). The coding sequence has been codon optimised for E. coli.
Notes
HBA and sfGFP are fused with a flexible linkerSource
The sequence was synthetized by GeneRay| Sequence Annotation | Location | Component / Role(s) |
| B0034 HBA-NsfGFP start stop B0010+B0012 | 2,13 20,1141 20,22 1139,1144 1154,1281 | ribosome_entry_site feature/rbs CDS feature/protein start_codon feature/start stop_codon feature/stop stem_loop feature/stem_loop |