BBa_K1093023

BBa_K1093023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1093023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakub Piątkowski
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:09:08

MinC/Lysis device-based kill switch



Types
DnaRegion

Roles
Other

sequence_feature

Sequences BBa_K1093023_sequence (Version 1)

Description

This brick combines elements from the well described bacterial lysis device (BBa_K1120808) with the gene coding for MinC protein (cell division inhibitor, characterised by Team Warsaw 2010). Addition of MinC is meant do reduce the odds of individual cells adapting to the device, thus making it suitable for the programmed cell death of bacterial population.
System is under the control of BBa_K098995 heat-inducible input device. The kill-switch is activated in 42 degrees Celsius.

Notes

Part is nearly identical to BBa_K1120808 regulated by BBa_K098995. The only difference is MinC coding sequence inserted before t gene

Source

The sequence was synthetized by GeneRay

Sequence Annotation Location Component / Role(s)
J23100
B0034
cIts
B0010+B0012
pR
B0034
MinC
t gene
e gene
B0010+B0012
J23100
antyholin
B0010
1,35
44,55
62,775
784,912
921,987
996,1007
1014,1709
1734,2390
2418,2912
2924,3095
3061,3095
3118,3414
3423,3502
promoter feature/promoter
feature/rbs ribosome_entry_site
CDS feature/protein
feature/stem_loop stem_loop
feature/promoter promoter
feature/rbs ribosome_entry_site
CDS feature/protein
CDS feature/protein
CDS feature/protein
feature/stem_loop stem_loop
promoter feature/promoter
feature/protein CDS
feature/stem_loop stem_loop
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1093023/1