Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakub Piątkowski
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:09:08
MinC/Lysis device-based kill switch
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K1093023_sequence (Version 1) |
Description
This brick combines elements from the well described bacterial lysis device (BBa_K1120808) with the gene coding for MinC protein (cell division inhibitor, characterised by Team Warsaw 2010). Addition of MinC is meant do reduce the odds of individual cells adapting to the device, thus making it suitable for the programmed cell death of bacterial population.System is under the control of BBa_K098995 heat-inducible input device. The kill-switch is activated in 42 degrees Celsius.
Notes
Part is nearly identical to BBa_K1120808 regulated by BBa_K098995. The only difference is MinC coding sequence inserted before t geneSource
The sequence was synthetized by GeneRay| Sequence Annotation | Location | Component / Role(s) |
| J23100 B0034 cIts B0010+B0012 pR B0034 MinC t gene e gene B0010+B0012 J23100 antyholin B0010 | 1,35 44,55 62,775 784,912 921,987 996,1007 1014,1709 1734,2390 2418,2912 2924,3095 3061,3095 3118,3414 3423,3502 | promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/protein feature/stem_loop stem_loop feature/promoter promoter feature/rbs ribosome_entry_site CDS feature/protein CDS feature/protein CDS feature/protein feature/stem_loop stem_loop promoter feature/promoter feature/protein CDS feature/stem_loop stem_loop |