BBa_K1093023

BBa_K1093023 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1093023

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jakub Piątkowski

Date created: 2013-09-22 11:00:00

Date modified: 2015-05-08 01:09:08

MinC/Lysis device-based kill switch

Details

• Types: DnaRegion
• Roles: Other; sequence_feature
• Sequences: BBa_K1093023_sequence (Version 1)

Description

This brick combines elements from the well described bacterial lysis device (BBa_K1120808) with the gene coding for MinC protein (cell division inhibitor, characterised by Team Warsaw 2010). Addition of MinC is meant do reduce the odds of individual cells adapting to the device, thus making it suitable for the programmed cell death of bacterial population.
System is under the control of BBa_K098995 heat-inducible input device. The kill-switch is activated in 42 degrees Celsius.

Notes

Part is nearly identical to BBa_K1120808 regulated by BBa_K098995. The only difference is MinC coding sequence inserted before t gene

Source

The sequence was synthetized by GeneRay

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
J23100	1,35	promoter, feature/promoter
B0034	44,55	feature/rbs, ribosome_entry_site
cIts	62,775	CDS, feature/protein
B0010+B0012	784,912	feature/stem_loop, stem_loop
pR	921,987	feature/promoter, promoter
B0034	996,1007	feature/rbs, ribosome_entry_site
MinC	1014,1709	CDS, feature/protein
t gene	1734,2390	CDS, feature/protein
e gene	2418,2912	CDS, feature/protein
B0010+B0012	2924,3095	feature/stem_loop, stem_loop
J23100	3061,3095	promoter, feature/promoter
antyholin	3118,3414	feature/protein, CDS
B0010	3423,3502	feature/stem_loop, stem_loop

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1093023/1

Member of these Collections

• iGEM Parts Registry