Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakub Piątkowski
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:09:08
MinC/Lysis device-based kill switch with delayed expression
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K1093024_sequence (Version 1) |
Description
This part combines elements from the well described bacterial lysis device (BBa_K1120808) with the gene coding for MinC protein (cell division inhibitor, characterised by Team Warsaw 2010). Addition of MinC is meant do reduce the odds of individual cells adapting to the device, thus making it suitable for the programmed cell death of bacterial population. In order amplify this effect, expression of lysis device is delayed in relation to MinC.System is under the control of BBa_K098995 heat-inducible input device. The kill-switch is activated in 42 degrees Celsius.
Notes
Delay is obtained with the use of LuxI/LuxR systemSource
The sequence was synthetized by GeneRay| Sequence Annotation | Location | Component / Role(s) |
| J23100 B0034 cIts B0034 LuxR B0010+B0012 pR B0034 MinC B0034 luxI B0010+B0012 pLux t gene e gene B0010+B0012 J23100 antyholin B0010 | 1,35 44,55 62,775 784,795 802,1557 1566,1694 1703,1769 1778,1789 1796,2491 2500,2511 2518,3132 3144,3272 3281,3335 3360,4016 4044,4538 4550,4678 4687,4721 4744,5040 5049,5128 | promoter feature/promoter ribosome_entry_site feature/rbs CDS feature/protein ribosome_entry_site feature/rbs CDS feature/protein feature/stem_loop stem_loop feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/protein ribosome_entry_site feature/rbs CDS feature/protein stem_loop feature/stem_loop promoter feature/promoter feature/protein CDS feature/protein CDS feature/stem_loop stem_loop feature/promoter promoter feature/protein CDS feature/stem_loop stem_loop |