Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakub Piątkowski
Date created: 2013-09-20 11:00:00
Date modified: 2015-05-08 01:09:08
HBB+CsfGFP m12 (+B0034)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1093031_sequence (Version 1) |
Description
Human hemoglobin beta chain fused at C-terminus with the C-terminal fragment of superfolder green fluorescent protein. m12 mutated version of the C-terminal sfGFP fragment offers larger specificity at the price of lowers sensitivity (as opposed to m6 vaersion) Along with BBa_K1093020 and BBa_K1093021 it is intended as a part of a reporter system for acrylamide detection. Formation of adducts on the N-terminal valine of both hemoglobin chains is known to inhibit proper assembly of hemoglobin tetramer. Part already contains a strong RBS (BBa_B0034). The coding sequence has been codon optimised for E. coli.Notes
HBB and sfGFP are fused with a flexible linker BBa_K157009Source
The sequence was synthetized by GeneRay| Sequence Annotation | Location | Component / Role(s) |
| B0034 HBB-CsfGFP (m12) start stop B0010+B0012 | 1,12 19,561 19,21 562,567 577,704 | feature/rbs ribosome_entry_site feature/protein CDS start_codon feature/start stop_codon feature/stop stem_loop feature/stem_loop |