BBa_K1093031

BBa_K1093031 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1093031
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakub Piątkowski
Date created: 2013-09-20 11:00:00
Date modified: 2015-05-08 01:09:08

HBB+CsfGFP m12 (+B0034)



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K1093031_sequence (Version 1)

Description

Human hemoglobin beta chain fused at C-terminus with the C-terminal fragment of superfolder green fluorescent protein. m12 mutated version of the C-terminal sfGFP fragment offers larger specificity at the price of lowers sensitivity (as opposed to m6 vaersion) Along with BBa_K1093020 and BBa_K1093021 it is intended as a part of a reporter system for acrylamide detection. Formation of adducts on the N-terminal valine of both hemoglobin chains is known to inhibit proper assembly of hemoglobin tetramer. Part already contains a strong RBS (BBa_B0034). The coding sequence has been codon optimised for E. coli.

Notes

HBB and sfGFP are fused with a flexible linker BBa_K157009

Source

The sequence was synthetized by GeneRay

Sequence Annotation Location Component / Role(s)
B0034
HBB-CsfGFP (m12)
start
stop
B0010+B0012
1,12
19,561
19,21
562,567
577,704
feature/rbs ribosome_entry_site
feature/protein CDS
start_codon feature/start
stop_codon feature/stop
stem_loop feature/stem_loop
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1093031/1