Types | DnaRegion
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Roles | engineered_region
Reporter
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Sequences | BBa_K1093031_sequence (Version 1)
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Description
Human hemoglobin beta chain fused at C-terminus with the C-terminal fragment of superfolder green fluorescent protein. m12 mutated version of the C-terminal sfGFP fragment offers larger specificity at the price of lowers sensitivity (as opposed to m6 vaersion) Along with BBa_K1093020 and BBa_K1093021 it is intended as a part of a reporter system for acrylamide detection. Formation of adducts on the N-terminal valine of both hemoglobin chains is known to inhibit proper assembly of hemoglobin tetramer. Part already contains a strong RBS (BBa_B0034). The coding sequence has been codon optimised for E. coli.
Notes
HBB and sfGFP are fused with a flexible linker BBa_K157009
Source
The sequence was synthetized by GeneRay