Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emil Christian Fischer
Date created: 2013-09-02 11:00:00
Date modified: 2015-05-08 01:09:08
Enhanced flavin-binding fluorescent protein (eFbFP)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1094000_sequence (Version 1) |
Description
The reporter eFbFP is a fluorescent protein that uses flavin mono nucleotide (FMN) as a cofacter. The reporter is suitable for use in anaerobic conditions. Excitation can be done with 485nm and emission peak can be measured at 500nm.Notes
Fluorescence is not easily spotted via fluorescence microscopy using GFP-filters. Thus, a 485nm filter is recommended.Source
Pseudomonas putida. Enhanced via F37S mutation.Originally described by:
Arnab Mukherjee, Kevin B Weyant, Joshua Walker and Charles M Schroeder (2012): Directed evolution of bright mutants of an oxygen-independent flavin-binding fluorescent protein from Pseudomonas putida. J. Mol. Eng., 6:20.