Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: YingQi Lou
Date created: 2013-09-12 11:00:00
Date modified: 2015-05-08 01:09:08
Csy4 generator with no terminator
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1100009_sequence (Version 1) |
Description
Csy4 is a member of CRISPR pathway discovered in Pseudomonas aeruginosa. It is a single endoRNase that recognizes and cleaves a 28-nucleotide repetitive sequence and produces stable transcripts with a 5???hydroxylgroup, which can eliminate unwanted interactions between 5???UTRs and translational elements such as RBSs to standardize the expression of the elements.Notes
There is no terminator here. Therefore, it can be add upstream of any terminator you are interested in. With a sfGFP reporter with a insulator -- the 16nt Csy4 cleavage loci, you can estimate how downstream sequence influences the terminator efficiency, like the study we did with the part BBa_K1100011.Source
The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 TetR 1 -35 -10 B0034 Insulator: Csy4 cleavage loci Csy4 | 1,54 1,19 20,25 43,48 79,89 62,77 96,659 | engineered_region feature/BioBrick non_covalent_binding_site feature/binding promoter feature/promoter feature/promoter promoter feature/rbs ribosome_entry_site stem_loop feature/stem_loop CDS feature/protein |