Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ting-Yun Chiang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:09
AhpCp2D1
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1104204_sequence (Version 1) |
Description
We improve the function of a BioBrick Part: AhpC promoter(K362001)designed by 2010 KIT-Tokyo team into four versions.On PartRegistry, the complex part(according to Ecocyc) composition contains hybrid promoters, shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG.This part contains 3 promoters: AhpC1, DsbG, AhpC2, 2 TFBSs for OxyR, and coding sequence in the initiate part(K362001)was deleted.
Notes
We delete the dsbG coding and following sequence next to dsbG reverse primer, making the functional sequence shorter, so that PCR can be more easy and efficient.Source
E. coli MG1655 genomic sequence| Sequence Annotation | Location | Component / Role(s) |
| OxyR binding site1 OxyR binding site2 (AhpCp2)-35 (AhpCp2)-10 ctgcag->ctacag g->a DsbGp OxyR binding site3 OxyR binding site4 (AhpCp1)-35 (AhpCp1)-10 | 118,137 141,157 166,171 192,197 233,238 235,235 285,309 356,372 378,394 393,398 417,422 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site promoter feature/promoter promoter feature/promoter sequence_feature feature/misc sequence_alteration feature/mutation feature/promoter promoter non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/promoter promoter promoter feature/promoter |