BBa_K1104205

BBa_K1104205 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1104205
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ting-Yun Chiang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:09

AhpCp2



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1104205_sequence (Version 1)

Description

We improve the function of a BioBrick Part: AhpC promoter(K362001)designed by 2010 KIT-Tokyo team into four versions.On PartRegistry, the complex part(according to Ecocyc) composition contains hybrid promoters(AhpC1, AhpC2, and DsbG promoter), shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG.
There is only AhpC2 promoter in this part. We designed the new part and test the promoter alone.

Notes

Because AhpC promoter(K362001) on PartRegistry is a hybrid promoter containing AhpC1, AhpC2, and DsbG promoter in its 1000bp, we design the new part: AhpC2 and test it apart.

Source

E. coli MG1655 genomic sequence

Sequence Annotation Location Component / Role(s)
OxyR binding site1
OxyR binding site2
(AhpCp2)-35
(AhpCp2)-10
118,134
141,157
166,171
192,197
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
promoter feature/promoter
feature/promoter promoter
igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1104205/1