Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Po-Hsi Lin
Date created: 2013-10-26 11:00:00
Date modified: 2015-05-08 01:09:10
Positive feedback produced by T7 system
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1104702_sequence (Version 1) |
Description
We created the circuit below. Constitutive promoter J23102 is cloned in the head of circuit. With reporter succeeding to the promoter, we could figure out whether the result was caused by promoter or not. And then there is the terminator we would test. After the terminator is the T7 polymerase which would open up the subsequent positive feedback. Besides, we used the other fluorescence protein as our second reporter. By testing the fluorescence from the second reporter, we could know how much proteins would be expressed on this site. Our control is the similar circuit without positive feedback below.Notes
.Source
.| Access | Instance | Definition |
| public public public public public public public public public | BBa_I712074 BBa_B0034 BBa_K145001 BBa_I13507 BBa_J23102 BBa_E0840 BBa_B0034 BBa_K145001 BBa_B0015 | BBa_I712074 BBa_B0034 T7pol BBa_I13507 BBa_J23102 GFP genera BBa_B0034 T7pol BBa_B0015 |