Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dr. ??zlem Darcansoy İşeri
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:09:10
LqsR promoter with RBS
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1109003_sequence (Version 1) |
Description
LqsR is the gene encoding response regulator component of the legionella quorum sensing (lqs). LqsR promoter region is controlled by its own response regulator LqsR. This composite part contains RBS downstream of the promoter region. When any ORF is cloned to the downstream of this composite part, it can be expressed in response to legionella auto-inducer (LAI-I).Notes
LqsR promoter part is a new biobrick with prefix and suffix regions, and RBS (BBa_B0034) was ligated to downstream of it.Source
Legionella pneumophila genomic lqs gene cluster was cloned into pNT-1 plasmid (Cellular Microbiology 9(12):2903-2920, 2007.) LqsR promoter region was amplified by PCR as a biobrick, and ligated with RBS (BBa_B0034).| Access | Instance | Definition |
| public public | BBa_K1109001 BBa_B0034 | BBa_K1109001 BBa_B0034 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1109001 BBa_B0034 | 1,266 275,286 | BBa_K1109001 BBa_B0034 |