Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Berkhan GEN??
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:09:10
lqs response unite
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K1109006_sequence (Version 1) |
Description
LqsR and LqsS are the genes encoding response regulator and sensor kinase components of the legionella quorum sensing (lqs) system, respectively. This composite part contains a constitutive promote region BBa_K823008 in the upstream these genes. When this composite ???lqs response unit??? is cloned into E. coli, the bacteria can express two components of the Legionella QS i.e; cell surface sensor kinase and cytosolic DNA binding component response regulator constitutively.Notes
The LqsR and LqsS genes have illegal restriction enzyme cutting sites inside their coding regions. So, BBa_K1109002 composite part was amplified with primers having SpeI cutting sites, and cloned into the downstream of a constiutive promoter in the pSB1C3.Source
Source of the subparts are the 2013 DNA Distribution Kit and pNT-1 plasmid(Cellular Microbiology 9(12):2903-2920, 2007.)| Access | Instance | Definition |
| public public | BBa_K823008 BBa_K1109002 | BBa_K823008 BBa_K1109002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K823008 BBa_K1109002 | 1,35 44,3292 | BBa_K823008 BBa_K1109002 |