Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sandra Elisabeth Chaudron, Caroline Desmurget and Mareike Apelt
Date created: 2013-10-28 12:00:00
Date modified: 2015-05-08 01:09:10
INP_Streptavidin_EYFP Fusion Protein
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1111016_sequence (Version 1) |
Description
This part is a Fusion protein made respectively of an Ice Nucleation Protein (INP), a Streptavidin protein and EYFP.Notes
Obtained by gibson assembly using PCR products from BBa_K283010 and BBa_K523013 Biobricks.Source
To be used in E. Coli| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter CAP binding site Lac LacZ BBa_K523013 INP INP start PCR added INP-Strep Linker Streptavidin start BBa_K283010 without stop codon Strep-EFYP Linker BBa_K523013 INP INP start INP stop T7 terminator Fusion Protein | 2,20 249,270 285,315 323,339 623,1555 623,625 1556,1573 1574,1576 1574,1957 1958,2008 2009,2778 2009,2011 2776,2778 2811,2838 623,2778 | feature/promoter promoter non_covalent_binding_site feature/binding feature/promoter promoter operator feature/operator feature/BioBrick engineered_region feature/start start_codon sequence_feature feature/misc start_codon feature/start engineered_region feature/BioBrick sequence_feature feature/misc feature/BioBrick engineered_region feature/start start_codon stop_codon feature/stop sequence_feature feature/misc CDS feature/protein |