BBa_K1112000

BBa_K1112000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1112000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pedro Luis Dorado Morales
Date created: 2013-09-09 11:00:00
Date modified: 2015-05-08 01:09:11

fadB promoter + flp-21 iRNA



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1112000_sequence (Version 1)

Description

This composite part consists of the antisense sequence of flp-21 transcript of C.elegans, placed under the control of the fatty acid-sensitive promoter fadA of E. coli.

Notes

Intron sequences of flp-21 gene were removed for the design of the iRNA.

Source

fadA promoter sequence comes from E. coli DC530 strain, whereas flp-21 gene sequence from C.elegans N2 strain was used as template for the design of the antisense RNA.

Sequence Annotation Location Component / Role(s)
fadB promoter
flp21 iRNA (antisense sequence of flp21 C. elegans mRNA)
Terminator
1,81
82,281
282,344
promoter feature/promoter
sequence_feature feature/dna
feature/stop stop_codon
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BBa_K1112000/1