BBa_K1112001

BBa_K1112001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1112001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alba Iglesias Vilches

Date created: 2013-09-09 11:00:00

Date modified: 2015-05-08 01:09:11

pGlnA + hmsHFRS operon

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1112001_sequence (Version 1)

Description

To control the expression of the operon, we used a promoter from the standard registry of parts, which was characterized by our team last year (see Part:BBa_K763003).

The molecular mechanism works as follows: with low nitrogen in the media, the promoter is activated and a biofilm of E.coli over C.elegans should be triggered and with high nitrogen concentration, the biobrick is unactive and Pseudomonas will not form the biofilm.

Notes

We developed a nitrogen-gradation tube experiment, which means that we added different (NH4)2SO4 concentrations to each tube, so we can measure a gradation of the protein fluorescence. We used a synthetic medium for this.

Source

The operon used comes from Xenorhabdus nematophila, a nematode-dependent parasite. This operon allows the formation of a biofilm on nematodes pathogenic to insects and it is thus key in this nematode-bacteria symbiotic association that kills insects.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pGlnA	1,210	promoter, feature/promoter
hmsHFRS operon (biofilm formation genes)	211,6341	feature/dna, sequence_feature
HmsH	211,2560	CDS, feature/protein
HmsF	2561,4597	CDS, feature/protein
HmsR	4598,5968	CDS, feature/protein
HmsS	5969,6341	CDS, feature/protein

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1112001/1

Member of these Collections

• iGEM Parts Registry