BBa_K1113502

BBa_K1113502 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1113502
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Belen Cespedes
Date created: 2013-09-17 11:00:00
Date modified: 2015-05-08 01:09:11

GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag



Types
DnaRegion

Roles
engineered_region

Reporter

Description

This part consist in the superfolder GFP (BBa_I746916) attached to two targeting sequences: The large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme (BBa_K1113701) which deliver into the carboxysome and the ssrA degradation tag (BBa_M0052) which is a targeting sequence for degradation by the protease ClpXP. This construct is used as a reporter for the correct assembly of the Carboxysome, it works with the competition of the two targeting sequence, in the case of a correct assembly of the carboxysome the microcompartment will ???protect??? the fluorescens, in any other case this will be degraded by the protease

Notes

This part it constructed with a mutation in the Prefix (from ???cggccgc??? to ???atacctg???) which erase the NotI restriction site, this modification allow the correct design of primers for Gibson assembly. This part its also constructed on the pSB4K5 standard vector

Source

The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley). All the rest are bricks available in the Registry

igem#experience
None
 
igem#sampleStatus
No part sequence
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1113502/1