BBa_K1114300

BBa_K1114300 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1114300
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Devina Desai
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:12

This is the MoClo formatted part of BBa_B0015.



Types
DnaRegion

Roles
terminator

Terminator

Sequences BBa_K1114300_sequence (Version 1)

Description

This is a Level 0 MoClo part with flanking sites D on the 5' side and site E on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_.

Notes

This is a Level 0 MoClo part with flanking sites D on the 5' side and site E on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_.

Source

iGEM kit

Sequence Annotation Location Component / Role(s)
MoClo Fusion Site D
MoClo Fusion Site E
BBa_B0015
1,4
134,137
5,133
sequence_feature feature/misc
feature/misc sequence_feature
feature/BioBrick engineered_region
igem#experience None  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K1114300/1