Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Devina Desai
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:12
This is a MoClo level 0 destination vector.
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1114401_sequence (Version 1) |
Description
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites B on the 5' side and site C on the 3' side of the part.The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
Notes
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites B on the 5' side and site C on the 3' side of the part.The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
Source
igem kit| Sequence Annotation | Location | Component / Role(s) |
| BbsI SpeI BsaI MoClo Fusion Site B BbsI MoClo Fusion Site C BsaI SpeI alpha fragment of lacZ | 509,514 1,6 8,13 15,18 21,26 517,520 522,527 529,534 27,508 | feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/cds CDS |