Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Devina Desai
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:12
This is a MoClo level 0 destination vector.
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1114403_sequence (Version 1) |
Description
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites C on the 5' side and site D on the 3' side of the part.The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
Notes
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites C on the 5' side and site D on the 3' side of the part.The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
Source
iGEM kit| Sequence Annotation | Location | Component / Role(s) |
| SpeI SpeI BsaI MoClo Fusion Site C BbsI BsaI MoClo Fusion Site D BbsI alpha fragment of lacZ | 1,6 529,534 8,13 15,18 21,26 522,527 517,520 509,514 27,508 | sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc CDS feature/cds |