Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Devina Desai
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:12
This is a MoClo level 0 destination vector.
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1114408_sequence (Version 1) |
Description
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites D on the 5' side and site F on the 3' side of the part.The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
Notes
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites D on the 5' side and site F on the 3' side of the part.The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.
Source
iGEM kit| Sequence Annotation | Location | Component / Role(s) |
| SpeI SpeI BsaI MoClo Fusion Site D BbsI SpeI BsaI MoClo Fusion Site F BbsI alpha fragment of lacZ | 1,6 7,12 14,19 21,24 27,32 535,540 528,533 523,526 515,520 33,514 | feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/cds CDS |