Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jake Awtry
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:09:12
Level 1 MoClo Destination Vector with F and G fusion sites
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1114420_sequence (Version 1) |
Description
Design Notes
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites F on the 5' side and site G on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; Y =TGTG.
Summary of modifications from original part:
Backbone is a modified version of pSB1K3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_pSB1K3.
Source
iGEM Distribution Kit
References
Notes
Design Notes
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites F on the 5' side and site G on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; Y =TGTG.
Summary of modifications from original part:
Backbone is a modified version of pSB1K3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_pSB1K3.
Source
iGEM Distribution Kit