Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jake Awtry
Date created: 2013-09-11 11:00:00
Date modified: 2015-05-08 01:09:12
MoClo Level 1 RFP reporter with AE fusion sites
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1114505_sequence (Version 1) |
Description
pJ04B16Rm_AE
This is a MoClo Level 1 reporting device containing the Level 0 parts J23104_AB, BCD16_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
Notes
pJ04B16Rm_AE
This is a MoClo Level 1 reporting device containing the Level 0 parts J23104_AB, BCD16_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
Source
Construct of MoClo Level 0 parts derived from iGEM Distribution Kits| Access | Instance | Definition |
| public public public public public | BBa_K1114005 BBa_K1114111 BBa_K1114211 BBa_K1114300 BBa_K1114418 | BBa_K1114005 BBa_K1114111 BBa_K1114211 BBa_K1114300 BBa_K1114418 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1114005 BBa_K1114111 BBa_K1114211 BBa_K1114300 BBa_K1114418 | 1,43 52,143 152,840 849,985 994,1512 | BBa_K1114005 BBa_K1114111 BBa_K1114211 BBa_K1114300 BBa_K1114418 |