Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jake Awtry
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:12
BioBrick adaptation of MoClo Level 1 RFP Reporter
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1114703_sequence (Version 1) |
Description
This is a BioBrick adaptation of a Level 1 MoClo device that constitutively expresses RFP. It contains the Level 0 parts J23104_AB, BCD13_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
Notes
This is a BioBrick adaptation of a Level 1 MoClo device that constitutively expresses RFP. It contains the Level 0 parts J23104_AB, BCD13_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
Source
Construct of MoClo Level 0 parts derived from the iGEM Distribution Kit| Access | Instance | Definition |
| public public public public | BBa_K1114005 BBa_K1114109 BBa_K1114211 BBa_K1114300 | BBa_K1114005 BBa_K1114109 BBa_K1114211 BBa_K1114300 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1114005 BBa_K1114109 BBa_K1114211 BBa_K1114300 | 1,43 44,135 136,824 825,961 | BBa_K1114005 BBa_K1114109 BBa_K1114211 BBa_K1114300 |