MLS

BBa_K1119000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1119000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wai In Hui

Date created: 2013-09-10 11:00:00

Date modified: 2015-05-08 01:09:13

Mitochondrial Leader Sequence in RFC10 standard

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K1119000_sequence (Version 1)

Description

Mitochondrial leader sequence (MLS) helps direct protein to the mitochondria. When this sequence is cloned in frame at the N-terminus of the protein, it can target the recombinant protein to the mitochondria in mammalian cells. MLS will be removed upon translocation into the mitochondrial matrix. However, at least four additional amino acids (Ile-His-Ser-Leu) on this sequence are known to be retained on the N-terminus of the protein.

Notes

No

Source

Plasmid from Invitrogen: pCMV/myc/mito (http://tools.lifetechnologies.com/content/sfs/manuals/pshooter_pcmv_man.pdf)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Mitochondrial Leader Sequence	1,87	feature/protein, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1119000/1

Member of these Collections

• //chassis/eukaryote/human
• //proteindomain/localization
• iGEM Parts Registry