Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lina Gasiūnaitė
Date created: 2013-08-12 11:00:00
Date modified: 2015-05-08 01:09:14
Genabler acceptor lacZα
cassette
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1122011_sequence (Version 1) |
Description
Vector pAcceptor2 consisting of pSB1C3 with the acceptor lacZ'alpha cassette is used in Genabler assembly.Notes
lacZ'alpha is flanked by outward-facing EarI sites to be compatible with Genabler assembly.Source
The lacZ'alpha cassette with outward-facing EarI sites was ordered from Life Technologies Corporation as GeneArt?? Strings??? DNA Fragments.| Sequence Annotation | Location | Component / Role(s) |
| EarI site EarI site CAP binding site -35 -10 lacI binding site rbs lacZ' | 5,10 370,375 17,54 66,71 89,94 101,121 128,131 139,369 | sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/promoter promoter feature/promoter promoter feature/misc sequence_feature feature/rbs ribosome_entry_site CDS feature/cds |