BBa_K112232

BBa_K112232 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112232

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bing Xia

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:15

rbs.barstar!

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K112232_sequence (Version 1)

Description

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Source

Construction of {rbs.barstar!} Biobrick Part K112232

PCR mea035/mea034 on Bacillus amyloliquefaciens gen          (320 bp, EcoRI/BamHI)

Sub into pBca1256                                            (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112232



mea035           Forward Biobricking of {rbs.barstar!}  

cgataGAATTCatgAGATCTcataagaaaggagccgcacatg

mea034           Reverse Biobricking of {rbs.barstar!}  

cgttaGGATCCttaagaaagtatgatggtgatgtcgcagcc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112232/1

Member of these Collections

• iGEM Parts Registry