Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aleksandra Lewicka, Jan Lyczakowski
Date created: 2013-08-29 11:00:00
Date modified: 2015-05-08 01:09:15
IPTG inducible pdc and adhB
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K1122676_sequence (Version 1) |
Description
This parts enables expression of pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) using IPTG induction. Those enzymes are involved in conversion of pyruvate to ethanol via an acetaldehyde intermediate. The pdc and adhB sequences come from Zymomonas mobilis however, they are codon optimised for E. coli.Notes
Presence of LacZ alpha peptide upstream of the ORFs coding for the enzymes enables blue- white screening.Source
synthetic| Access | Instance | Definition |
| public public | BBa_J33207 BBa_K173003 | BBa_J33207 BBa_K173003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33207 BBa_K173003 | 1,600 609,3660 | BBa_J33207 BBa_K173003 |