BBa_K1123000

BBa_K1123000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1123000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ardjan van der Linden
Date created: 2013-08-30 11:00:00
Date modified: 2015-05-08 01:09:15

FNR Promoter



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1123000_sequence (Version 1)

Description

This part is a standard FNR promoter. It has two FNR binding sites at -41.5 and at -91.5 with 0 being the transcriptional starting point of this promoter.
The promoter can be used in E.coli strains to induce protein expression in anaerobic environments. It is natively used to induce metabolic processes when the E.coli bacteria enter anaerobic environments.
The part can be used to express any protein sequence placed behind it.

Notes

To remove the EcoRI site we adapted the tail of the sequence which we had found in the above mentioned article. The EcoRI site was situated in at -132. We therefore removed the sequence from -132 to -116 and replaced it with a sequence of 4 base pairs namely: GGTA. We hoped that this adaptation would be of no influence on the efficiency of the promoter.

Source

Part sourced from the following study into the FNR promomoter:
"Transcriptional Regulation by Tandem-Bound FNR at Escherichia coli Promoters", Barnard et al.

igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K1123000/1