BBa_K1123002

BBa_K1123002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1123002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ardjan van der Linden
Date created: 2013-09-07 11:00:00
Date modified: 2015-05-08 01:09:15

1ETF Protein Production Construct



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1123002_sequence (Version 1)

Description

This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the 1ETF Protein sequence. The FNR promotor sequence enables us to bring the 1ETF protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The 1ETF protein is a HIV protein which we are expressing with the sole purpose of using it as a CEST contrast agent.

Notes

No real considerations were made in the construction of this composite part. The main point of focus was the compatibility of each of the parts with each other and the E.coli bacteria. Furthermore we then had to ensure that the protein sequence was in frame with the promotor to ensure that expression would be performed correctly.

Source

The composite designed was something we devised ourselves although each of the separate parts was sourced from a different area. The sources for each of the relevant parts can be found on their respective pages.

igem#experience None  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K1123002/1