Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ardjan van der Linden
Date created: 2013-09-07 11:00:00
Date modified: 2015-05-08 01:09:15
P(RG) Protein Production Construct
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1123006_sequence (Version 1) |
Description
This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the Poly(Arginine-Glycine) (P(RG)) Protein sequence. The FNR promotor sequence enables us to bring the P(RG) protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The P(RG) protein is a protein containing 216 repeats od the Arginine-Glycine amino acid combination designed by us ourselves. It is being expressed with the sole purpose of using it to provide CEST contrast in an MRI.Notes
No real considerations were made in the construction of this composite part. The main point of focus was the compatibility of each of the parts with each other and the E.coli bacteria. Furthermore we then had to ensure that the protein sequence was in frame with the promotor to ensure that expression would be performed correctly.Source
The composite designed was something we devised ourselves although each of the separate parts was sourced from a different area. The sources for each of the relevant parts can be found on their respective pages.| Access | Instance | Definition |
| public public public public public | BBa_K1123000 BBa_K1123010 BBa_K1123012 BBa_K1123011 BBa_B1006 | BBa_K1123000 BBa_K1123010 BBa_K1123012 BBa_K1123011 BBa_B1006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1123000 BBa_K1123010 BBa_K1123012 BBa_K1123011 BBa_B1006 | 1,120 121,142 143,205 638,680 681,719 | BBa_K1123000 BBa_K1123010 BBa_K1123012 BBa_K1123011 BBa_B1006 |