BBa_K1123006

BBa_K1123006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1123006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ardjan van der Linden
Date created: 2013-09-07 11:00:00
Date modified: 2015-05-08 01:09:15

P(RG) Protein Production Construct



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1123006_sequence (Version 1)

Description

This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the Poly(Arginine-Glycine) (P(RG)) Protein sequence. The FNR promotor sequence enables us to bring the P(RG) protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The P(RG) protein is a protein containing 216 repeats od the Arginine-Glycine amino acid combination designed by us ourselves. It is being expressed with the sole purpose of using it to provide CEST contrast in an MRI.

Notes

No real considerations were made in the construction of this composite part. The main point of focus was the compatibility of each of the parts with each other and the E.coli bacteria. Furthermore we then had to ensure that the protein sequence was in frame with the promotor to ensure that expression would be performed correctly.

Source

The composite designed was something we devised ourselves although each of the separate parts was sourced from a different area. The sources for each of the relevant parts can be found on their respective pages.

igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1123006/1