BBa_K112308

BBa_K112308 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112308

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:16

{lambda holin >}
adheres to Berkeley standard

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K112308_sequence (Version 1)

Description

lambda holin with start and no stop codon in BBb format.
More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR al0007/al0010 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112308



al0007	Forward Biobricking of lambda holin with start and no stop codon

CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg

al0010	Reverse Biobricking of lambda holin wtih start and no stop codon

CGttaGGATCCttgatttctaccatcttctactccggc

Source

pSB3C6-Bjh1502

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112308/1

Member of these Collections

• //cds/membrane/lysis
• //function/celldeath
• iGEM Parts Registry