BBa_K112308

BBa_K112308 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112308
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aron Lau
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:16

{lambda holin >}
adheres to Berkeley standard



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_K112308_sequence (Version 1)

Description

lambda holin with start and no stop codon in BBb format.
More information about the BBb Format is available at:

BBb Standard Description Page

Notes


PCR al0007/al0010 on pSB3C6-Bjh1502 (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112308


al0007 Forward Biobricking of lambda holin with start and no stop codon
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0010 Reverse Biobricking of lambda holin wtih start and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc


Source

pSB3C6-Bjh1502

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K112308/1