BBa_K112309

BBa_K112309 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112309
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aron Lau
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:16

{< lambda holin >}
adheres to Berkley standard



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_K112309_sequence (Version 1)

Description

lambda holin with no start and stop codon in BBb format.
More information about the BBb Format is available at:

BBb Standard Description Page

Notes


PCR al0009/al0010 on pSB3C6-Bjh1502 (343bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112309


al0009 Forward Biobricking of lambda holin with no start and stop codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0010 Reverse Biobricking of lambda holin with no start and stop codon
CGttaGGATCCttgatttctaccatcttctactccggc


Source

pSB3C6-Bjh1502

igem#experience None  
igem#sampleStatus It's complicated  
igem#status Planning  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K112309/1