BBa_K112315

BBa_K112315 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112315

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:16

{< lambda antiholin >}
adheres to Berkeley standard

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K112315_sequence (Version 1)

Description

lambda antiholin with no start and stop codons. This part is actually in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR al0014/al0010 on pSB3C6-Bjh1503     (349bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112315



al0014	Forward Biobricking of lambda antiholin with no start and stop codons

CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg

al0010	Reverse Biobricking of lambda antiholin with no start and stop codons

CGttaGGATCCttgatttctaccatcttctactccggc

Source

pSB3C6-Bjh1503

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112315/1

Member of these Collections

• //cds/membrane/lysis
• //function/celldeath
• iGEM Parts Registry