BBa_K112316

BBa_K112316 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112316

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:16

{a~lambda antiholin!}
adheres to Berkeley standard

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K112316_sequence (Version 1)

Description

lambda antiholin ready for rbs addition and has stop codon. This part is actually in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR al0015/al0008 on pSB3C6-Bjh1503     (359bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112316



al0015	Forward Biobricking of lambda antiholin with spacer

CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc

al0008	Reverse Biobricking of lambda antiholin with spacer

CGttaGGATCCttattgatttctaccatcttctactccggc

Source

pSB3C6-Bjh1503

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112316/1

Member of these Collections

• //cds/membrane/lysis
• //function/celldeath
• iGEM Parts Registry